PUMA mediated afatinib-induced apoptosis in glioma cells

Introduction Afatinib exhibits a tumor-inhibiting effect in different cancers by inducing apoptosis; however, its pro-apoptosis role glioma cells is still not fully understood. Material and methods In the current study, two cell lines (U373-MG U87-MG) were treated with afatinib to measure their tolerance for afatinib-triggered death apoptosis. Results We found that treatment repressed both growth proliferation induced apoptosis cells. Moreover, it increased expression of pro-apoptotic p53-upregulated modulator (PUMA). The influence PUMA on was assessed overexpression knockdown lines. resulted an sensitivity toward afatinib, whereas abated Similarly, vivo potency U373-MG xenograft tumors wild type (WT) nude mice measured. reduced weight volume WT but did have same tumors. also significant Conclusions conclusion, induces mediating expression. This study warrants further investigation into mechanism glioblastoma treatment.